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KMID : 0385520130260060357
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Analytical Science & Technology
2013 Volume.26 No. 6 p.357 ~ p.363
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Separation and identification of selenoproteins in selenium-enriched yeast
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Kim Kyong-Mi
Pak Yong-Nam
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Abstract
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Selenium-containing proteins were separated from selenium-enriched yeast (SEY) using Trizol® reagent followed by anion exchange (AE) chromatography. This method is simpler and less time consuming than electrophoresis. Five selenium containing proteins were identified by on-line AE HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry). Each protein was enzymatically hydrolyzed to seleno-amino acids and separated with RP (reverse phase) HPLC for the identification of selenoproteins.
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KEYWORD
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Se-enriched yeast, HPLC-ICP/MS, selenoprotein, Trizol® reagent, anion exchange chromatography
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